Journal: Genetics

Article Title: A tale of two serines: the effects of histone H2A mutations S122A and S129A on chromosome nondisjunction in Saccharomyces cerevisiae

doi: 10.1093/genetics/iyae194

Figure Lengend Snippet: Example of CGH microarray from hta1 -S122A strain (SGK177-9). The strain was a diploid formed by a cross of haploids isogenic with W303 and S288c. DNA isolated from the euploid control haploid W303 was labeled with Cy3 dUTP and the DNA from the experimental sample was labeled with Cy5 dUTP, and these samples were hybridized in competition to microarrays containing oligonucleotides that cover the entire yeast genome (details in Materials and Methods ). The scanned microarray shows the ratio of hybridization for each chromosome. The red and green colors show significant relative increases and decreases of hybridization in the experimental sample vs the control sample. The light gray lines above each chromosome are log 2 transforms of the ratio with the baseline having a value of 0, the first line above the baseline indicating a value of 1 and so on. Thus, euploid chromosomes have a log 2 value of 0, trisomic chromosomes a log 2 value of 0.6, and tetrasomic chromosomes a value of 1. The data of isolate SGK177-9 show that the experimental strain is monosomic for chromosome VIII and trisomic for chromosomes III and X. “Spikes” of the red signal are telomere-associated sequences present in the diploid starting strain that are absent in the control strain.

Article Snippet: In brief, DNA from the control euploid strain W303-1A ( Matheson et al . 2017 ) was labeled with Cy3 dUTP and DNA from the experimental DNA was labeled with Cy5 dUTP.

Techniques: Microarray, Isolation, Control, Labeling, Hybridization